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+ container
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- claim New mitochondria arise from the growth and division of preexisting mitochondria, rather than forming de novo.
+ idea New mitochondria arise from the growth and division of preexisting mitochondria, rather than forming de novo.
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- prediction
+ predictions
- Two distinct populations would appear - one labeled, one unlabeled
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- method
+ experiment
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- method
- 1. Culture Neurospora strain requiring choline in logarithmic growth phase
- 2. Supply radioactively labeled choline (membrane precursor)
- 3. Transfer to non-radioactive medium
- 4. At intervals, isolate mitochondria
- 5. Spread mitochondria on slides for autoradiography
- 6. Repeat similar experiment with Tetrahymena using tritiated thymidine to label mitochondrial DNA
- 7. Track label distribution for four generations
+ method
+ 1. Culture Neurospora strain requiring choline in logarithmic growth phase
+ 2. Supply radioactively labeled choline (membrane precursor)
+ 3. Transfer to non-radioactive medium
+ 4. At intervals, isolate mitochondria
+ 5. Spread mitochondria on slides for autoradiography
+ 6. Repeat similar experiment with Tetrahymena using tritiated thymidine to label mitochondrial DNA
+ 7. Track label distribution for four generations
- prediction
- If mitochondria form de novo:
- - Two distinct populations would appear - one labeled, one unlabeled
- If mitochondria divide from existing ones:
- - Label would be uniformly distributed among all mitochondria
+ prediction
+ If mitochondria form de novo:
+ - Two distinct populations would appear - one labeled, one unlabeled
+ If mitochondria divide from existing ones:
+ - Label would be uniformly distributed among all mitochondria
- results
- Neurospora experiment:
- - Label was uniformly distributed among all mitochondria
- Tetrahymena experiment:
- - Label remained evenly distributed among all mitochondria through four generations of growth
+ results
+ Neurospora experiment:
+ - Label was uniformly distributed among all mitochondria
+ Tetrahymena experiment:
+ - Label remained evenly distributed among all mitochondria through four generations of growth
- conclusion
- The uniform distribution of labels in both experiments supports the hypothesis that new mitochondria arise from growth and division of preexisting mitochondria. The persistence of evenly distributed label through multiple generations particularly strengthens this conclusion by showing consistent inheritance of mitochondrial components.
+ conclusion The uniform distribution of labels in both experiments supports the hypothesis that new mitochondria arise from growth and division of preexisting mitochondria. The persistence of evenly distributed label through multiple generations particularly strengthens this conclusion by showing consistent inheritance of mitochondrial components.
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- hypothesisParser
+ claimParser
- cue hypothesis
+ cue claim
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- proof
- hypothesis
- New mitochondria arise from the growth and division of preexisting mitochondria, rather than forming de novo.
+ claim New mitochondria arise from the growth and division of preexisting mitochondria, rather than forming de novo.
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+ proof.parsers
+
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+ proof
+ hypothesis
+ New mitochondria arise from the growth and division of preexisting mitochondria, rather than forming de novo.
+
+ method
+ 1. Culture Neurospora strain requiring choline in logarithmic growth phase
+ 2. Supply radioactively labeled choline (membrane precursor)
+ 3. Transfer to non-radioactive medium
+ 4. At intervals, isolate mitochondria
+ 5. Spread mitochondria on slides for autoradiography
+ 6. Repeat similar experiment with Tetrahymena using tritiated thymidine to label mitochondrial DNA
+ 7. Track label distribution for four generations
+
+ prediction
+ If mitochondria form de novo:
+ - Two distinct populations would appear - one labeled, one unlabeled
+ If mitochondria divide from existing ones:
+ - Label would be uniformly distributed among all mitochondria
+
+ results
+ Neurospora experiment:
+ - Label was uniformly distributed among all mitochondria
+ Tetrahymena experiment:
+ - Label remained evenly distributed among all mitochondria through four generations of growth
+
+ conclusion
+ The uniform distribution of labels in both experiments supports the hypothesis that new mitochondria arise from growth and division of preexisting mitochondria. The persistence of evenly distributed label through multiple generations particularly strengthens this conclusion by showing consistent inheritance of mitochondrial components.
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